A Work-Shop on Stem Cells
June 23, 2006
Graduate College S. Chiara

9.00 Welcome of Prof. Silvano Focardi, Rector of Siena University

9.20 Welcome of Prof. Marco Ferrari, Director of School of Ph D in ‘Biotechnologies’ (Siena University)

9.30 Prof. Sergio Romagnani (Florence University): “New Acquisitions on Stem Cells from Human Adult Subjects”

10.10 Prof. David Kaplan (Tufts University-Boston): “Scaffold Designs to Direct Stem Cells toward Functional Tissue Outcomes”

10.40-11.00 Breach

11.00 Prof. Jonathan Garlik (Tufts University-Boston): “The Biology of 3D Human Skin Equivalents; Discovery, Development and Translation in the third Dimension”

11.40 Prof. Salvatore Oliviero (Siena University): “Genes Involved in the Growth and Differentiation of Embryonic Stem Cells toward Endothelial Cells”

12.30 Discussion

13.00 Light Lunch

14.30 Prof. Vincenzo Sorrentino (Siena University): "Human adult stem cells: from basic questions to applied answers“

15.10 Prof. Anne Poliard (Paris V University): “Dental Pulp Stem Cells”

16.00 Prof. Michel Goldberg (Paris V University): “How Extracellular Matrix Molecules Can stimulate the Proliferation and Differentiation of Pulp Stem Cells”

16.30 Prof. Marco Ventura (Siena University): “Bioethics in Stem Cell Research: Obstacle or Asset?”

17.00 Prof. Mario Marzilli (Siena University): " Biological cardiac repair?"

Emails of the Speakers
s.romagnani@DMI.UNIFI.IT
apoliard@usf.cnrs.fr
MGoldOd@aol.com
Ventura4@unisi.it
rettore@unisi.it
m.ferrari@mclink.it
sorrentino@unisi.it
oliviero@unisi.it
Jonathan.Garlick@tufts.edu
david.kaplan@tufts.edu
marzilli@unisi.it

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Prof. Garlick - Prof. Kaplan
June 22 - 26 -27, 2006
Centro Didattico
Policlinico Le Scotte

LIST OF TOPICS FOR 16 HOUR COURSE IN EPITHELIAL BIOLOGY AND HUMAN TISSUE ENGINEERING

1. Understanding the Tissue:

a. The Cellular Biology of Stratified Squamous Epithelia (2.5 hours)

b. The Biology of Epithelial Stem Cells and Their Basement Membrane Niche (2.5 hours)

c. The Biology of Wound Repair: the Activated Keratinocyte (2.5 hours)

2. Building the Tissue

a. Principles in the Construction of 3D Tissue Equivalents of Stratified Squamous Epithelium (2.5 hours)

b. 3D Tissue Models of Squamous Cell Carcinoma (2.5 hours)

c. 3D Tissue Biology as a Platform for Discovery: Translation from Bench to Bedside (2.5 hours)

 

 

 

 

 

 

 

 

 

2005

New applications in Confocal microscopy

Lectures:
Prof. Rainer Pepperkok
Advanced Light Microscopy Facility
EMBL– Heidelberg, Germany

Practice:
Timo Zimmerman, EMBL-Heidelberg, Germany
Maurizio Orlandini, Alessio Zippo, University of Siena

April 4-7 at 9,30 AM
Seminar room
Dipartimento di Biologia molecolare
via Fiorentina 1
Siena

Monday April 4th

Seminar 9,30 AM (90 min)
Title: Basic fluorescence microscopy
Topics covered: Fluorescence, epifluorescence microscopy, filters, fluorescent dyes, multicolour immunofluorescence, detectors for fluorescence, spectral imaging and linear unmixing.

Practical 3 PM (3-4h)
Title: Basics in fluorescence microscopy and digital imaging
Topics covered: set-up of epifluorescent microscopes, image acquisition, filter settings, multicolour imaging, quantitative imaging, troubleshooting.

Tuesday April 5th

Seminar 9,30 AM (90 min)
Title: 3D and 4D fluorescence microscopy
Topics covered: Confocal microscopy concepts, TIRF, deconvolution, confocal microscope systems and their characteristics, 3D and 4D image data reconstruction, analysis and presentation, ins and outs of imaging of living cells, labeling of proteins in living cells, cell biological applications.

Practical 3 PM (3-4h)
Title: Confocal microscopy in fixed and living cells
Topics covered: Introduction to multicolour Laser Scanning Microscope, 3D data acquisition, reconstruction, analysis and presentation.

Wednesday April 6th

Seminar 9,30 AM (90 min)
Title: Imaging biochemical reactions in fixed and living cells
Topics covered: Basics in fluorescence resonance energy transfer (FRET), methods to image FRET, sensor design, fluorescence recovery after photobleaching (FRAP) and related techniques, fluorescence correlation spectroscopy (FCS), applications.

Practical 3 PM (3-4h)
Title: FRET and FRAP measurements
Topics covered: Measuring FRET and FRET on a confocal microscope.

Thursday April 7th

Seminar 9,30 AM (60 min)
Title: High Content Screening Microscopy
Topics covered: Automated image data acquisition, strategies for robust assay design, image analysis

Seminar 11 AM (60 min)
Title: Studying membrane traffic by advanced imaging methods
Topics covered: Applications in membrane traffic

Practical 3 PM (3h)
Title: Basic concepts in image analysis
Topics covered: Image enhancement, quantification of fluorescence, automated object recognition, ImageJ.