ENVIRONMENTAL SCIENCE
Università di Siena Dipartimento Scienze Ambientali
Protein analysis
Electrophoresis (1-D, 2-D)
Immunoblotting
Automated purification of biologically active proteins and peptides by AKTA Purifier System
Purification of aminoacids and peptides by HPLC techniques
A monoclonal antibody production unit is also available
--------------------------------------------------------------------------------
DNA analysis
DNA, RNA extraction and mRNA purification from plant tissues
non-radioactive hybridization protocols for Southern, Northern, colony screening and selection
cloning into E. coli
non-radioactive cycle sequencing and silver staining
PCR
automated sequencing and DNA fragment analysis
DNA fingerprinting (RAPDs, AFLPs, Microsatellite isolation and amplification)
--------------------------------------------------------------------------------
Microscopy
Transmission electron microscopy (for chemically- and - freeze-substituted samples)
Scanning electron microscopy
Confocal Scanning microscopy
Video microscopy
Immunofluorescence and immunogold labeling techniques are available
Microinjection